ELISA Kit for Free Triiodothyronine (fT3)

ELISA Kit for Free Triiodothyronine (fT3)

Marca: Cloud Clone

Catálogo: CEA186Ge

Faixa de Detecção: 740.7-60000fg/mL

Aplicação: ELISA

Espécie: Pan-species (General)

Sensibilidade: The minimum detectable dose of this kit is typically less than 249.5fg/mL

Duração do ensaio: 2h

Método: Competitive Inhibition

Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to T3 has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled T3 analogues and unlabeled antigen (Standards or samples) with the pre-coated antibody. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate