ELISA Kit for Hydroxyproline (Hyp)

ELISA Kit for Hydroxyproline (Hyp)

Marca: Cloud Clone

Catálogo: CEA621Ge

Faixa de Detecção: 61.7-5,000ng/mL

Aplicação: ELISA

Espécie: Pan-species (General)

Sensibilidade: The minimum detectable dose of this kit is typically less than 24.3ng/mL

Duração do ensaio: 2h

Método: Competitive Inhibition

Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Hydroxyproline (Hyp) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Hydroxyproline (Hyp) and unlabeled Hydroxyproline (Hyp) (Standards or samples) with the pre-coated antibody specific to Hydroxyproline (Hyp). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added