ELISA Kit for Ubiquitin (Ub)

ELISA Kit for Ubiquitin (Ub)

Marca: Cloud Clone

Catálogo: CEA164Mi

Faixa de Detecção: 4.94-400ng/mL

Aplicação: ELISA

Espécie: Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit),

Sensibilidade: The minimum detectable dose of this kit is typically less than 1.92ng/mL

Duração do ensaio: 2h

Método: Competitive Inhibition

Descrição Protocolo: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Ubiquitin (Ub) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Ubiquitin (Ub) and unlabeled Ubiquitin (Ub) (Standards or samples) with the pre-coated antibody specific to Ubiquitin (Ub). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well